Leica NCL-L-SP-A Model Vehicle User Manual

Novocastra Liquid

Mouse Monoclonal Antibody

Surfactant Protein A

Product Code: NCL-L-SP-A

Leica Biosystems Newcastle Ltd

Balliol Business Park West

Benton Lane

Newcastle Upon Tyne NE12 8EW

United Kingdom

( +44 191 215 4242

Instructions for Use

Please read before using this product.

Check the integrity of the packaging before use.

Novocastra Liquid Mouse Monoclonal Antibody

Surfactant Protein A

Product Code: NCL-L-SP-A

Intended Use

For in vitro diagnostic use.

NCL-L-SP-A is intended for the qualitative identification by light microscopy of human surfactant protein A in paraffin sections. The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patients clinical history and other diagnostic tests by a qualified pathologist.

NCL- L- SP-A is recommended for the assessment of surfactant protein A in normal and neoplastic tissue.

Summary and Explanation

The first immunohistoperoxidase technique was reported by Nakane and Pierce.1 Since then many developments have occurred, leading to increased sensitivity over earlier techniques. A recent development has been the use of polymeric labeling. This technology has been applied to both primary antibodies2 and detection systems. The Novolink Polymer Detection Systems utilize a novel controlled polymerization technology to prepare polymeric HRP-linker antibody conjugates. Therefore, the problem of non-specific staining that can occur with Streptavidin/Biotin detection systems due to endogenous biotin does not occur.

Principle of Procedure

Immunohistochemical (IHC) staining techniques allow for the visualization of antigens via the sequential application of a specific antibody to the antigen (primary antibody), a secondary antibody to the primary antibody and an enzyme complex with a chromogenic substrate with interposed washing steps. The enzymatic activation of the chromogen results in a visible reaction product at the antigen site. The specimen may then be counterstained and coverslipped. Results are interpreted using a light microscope and aid in the differential diagnosis of pathophysiological processes, which may or may not be associated with a particular antigen.

NCL-L-SP-A is a liquid tissue culture supernatant containing sodium azide as a preservative.

Prokaryotic recombinant protein corresponding to the 104246 amino acid region of the human prosurfactant A molecule.

Specificity

Human surfactant protein A (prosurfactant A).

IgG2a, kappa

Total Protein Concentration

Total Protein

Refer to vial label for lot specific total protein concentration.

Antibody Concentration

Greater than or equal to 35 mg/L as determined by ELISA. Refer to vial label for lot specific Ig concentration.

Warnings and Precautions

This reagent has been prepared from the supernatant of cell culture. As it is a biological product, reasonable care should be taken when handling it.

This reagent contains sodium azide. A Material Safety Data Sheet is available upon request or available from

Consult federal, state or local regulations for disposal of any potentially toxic components.

Specimens, before and after fixation, and all materials exposed to them, should be handled as if capable of transmitting infection and disposed of with proper precautions.3 Never pipette reagents by mouth and avoid contacting the skin and mucous membranes with reagents and specimens. If reagents or specimens come in contact with sensitive areas, wash with copious amounts of water. Seek medical advice.

Minimize microbial contamination of reagents or an increase in non-specific staining may occur.

Incubation times or temperatures, other than those specified, may give erroneous results. Any such changes must be validated by the

Storage and Stability

Store at 28 C. Do not freeze. Return to 28 C immediately after use. Do not use after expiration date indicated on the vial label.

Storage conditions other than those specified above must be verified by the user.

The signs indicating contamination and/or instability of NCL-L-SP-A are: turbidity of the solution, odor development, and presence of precipitate.

Specimen Preparation

The recommended fixative is 10% neutral-buffered formalin for paraffin-embedded tissue sections.

Recommendations On Use

Immunohistochemistry on paraffin sections.

Heat Induced Epitope Retrieval (HIER): Please follow the instructions for use in Novocastra Epitope Retrieval Solution pH 9.

Suggested dilution: 1:2000 for 30 minutes at 25 C. This is provided as a guide and users should determine their own optimal working dilutions.

Visualization: Please follow the instructions for use in the Novolink Polymer Detection Systems. For further product information or support,