LEICA NCL-CK17 Dollhouse user manual

Novocastra Lyophilized

Mouse Monoclonal Antibody

Cytokeratin 17

Product Code: NCL-CK17

Intended Use

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

Specificity

Human cytokeratin 17 intermediate filament protein.

Antigen Used for

Immunizations

Keratin preparation from rat enterocytes.

Hybridoma Partner

Mouse myeloma (X63-Ag8.653).

Preparation

Lyophilized tissue culture supernatant containing sodium azide.

Reconstitute with the volume of sterile distilled water indicated on the vial label.

Effective on Frozen Tissue

Effective on Paraffin Wax

Embedded Tissue

Recommendations on Use

Immunohistochemistry on paraffin sections.

Heat Induced Epitope Retrieval (HIER): Please follow the instructions for use in Novocastra

Epitope Retrieval Solution pH6.

Suggested dilution: 1:20 for 30 minutes at 25 C. This is provided as a guide and users should determine their own optimal working dilutions.

Visualization: Please follow the instructions for use in the Novolink Polymer Detection Systems.

For further product information or support, contact your local distributor or regional office of Leica

The performance of this antibody should be validated when utilized with other manual staining systems or automated platforms.

Positive Controls

Immunohistochemistry: Trachea.

Staining Pattern

Cytoplasmic.

Storage and Stability

Store unopened antibody at 28 C. Under these conditions, there is no significant loss in product performance up to the expiry date indicated on the vial label. Do not use after expiration date indicated on the vial label. The reconstituted antibody is stable for at least two months when stored at 28 C. For long term storage, it is recommended that aliquots of the reconstituted antibody are stored frozen at -20 C (frost-free freezers are not recommended). Repeated freezing and thawing must be avoided. Prepare working dilutions on the day of use. Return to 28

C immediately after use. Storage conditions other than those specified above must be verified by the user.

Warnings and Precautions

This reagent has been prepared from the supernatant of cell culture confirm. As it is a biological product, reasonable care should be taken when handling it. This reagent contains sodium azide.

A Material Safety Data Sheet is available upon request or available from

Leica Biosystems Newcastle Ltd

Balliol Business Park West

Benton Lane

Newcastle Upon Tyne NE12 8EW

United Kingdom

( +44 191 215 4242

Western Blotting: HeLa cell line.

Leica Biosystems Newcastle Ltd NCL- Rev D CK17 21/01/2014

General Overview

NCL-CK17 reacts with the human cytokeratin intermediate filament protein (46 kD) identified as cytokeratin 17. In normal tissues, NCL-CK17 is reported to react mainly with basal cells of complex epithelia; basal cells of pseudostratified epithelium in the trachea, larynx and bronchi.

The antibody also reportedly reacts with myoepithelial cells in salivary glands and sweat glands.

General References

Markey A C, Lane E B, MacDonald D M, et al.. British Journal of Dermatology. 126: 154160

Troyanovsky S M, Guelstein V I, Tchipysheva T A, et al.. Journal of Cell Science. 93: 419426

Guelstein V I, Tchipysheva T A, Ermilova V D, et al.. International Journal of Cancer.42: 147153

Leica Biosystems Newcastle Ltd NCL- Rev D